KMID : 0381120140360010087
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Genes and Genomics 2014 Volume.36 No. 1 p.87 ~ p.93
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Triglyceride enhances susceptibility to TNF-¥á-induced cell death in THP-1 cells
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Lim Jae-Won
Kim Yoon-Suk Kim Sung-Hoon Cho Yoon-Jung Lee Min-Ho Jung Byung-Chul Jung Dong-ju Rhee Ki-Jong
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Abstract
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Monocytes and macrophages play a major role in atherosclerosis development. Previously, we found that triglyceride (TG) promoted cell death of PMA-differentiated THP-1 macrophages. In this study, we compared the responsiveness of THP-1 monocytes and PMA-differentiated THP-1 macrophages to TNF-¥á-induced cell death. We found that, whereas THP-1 monocytes were TNF-¥á-resistant, THP-1 macrophages were sensitive to TNF-¥á-induced cell death. THP-1 monocytes treated with TG underwent cell death beginning at 24 h and addition of TNF-¥á further increased cell death. Based on these observations, we hypothesized that TG-induced differentiation of THP-1 monocytes into THP-1 macrophages, subsequently allowing sensitivity to TNF-¥á. To determine if TG could induce differentiation of THP-1 monocytes into THP-1 macrophages, we examined the mRNA expression levels of the macrophage-specific markers, CD11b, CD18, CD36 and CD68, by RT-PCR analysis. Our results show that expression of CD11b, CD36 and CD68 increased in TG-treated THP-1 monocytes in a dose- and time-dependent manner; furthermore, TNF-¥á expression was upregulated in TG-treated THP-1 monocytes. We have concluded that TG induces differentiation of THP-1 monocytes into macrophages concomitant with the production of TNF-¥á and increased sensitivity to TNF-¥á-dependent cell death.
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KEYWORD
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THP-1 cells, Triglyceride, TNF-¥á, Cell viability
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